Analytical Data
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Gene name
USP47
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简介
The USP47 protein is a ubiquitin-specific protease that deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizes POLB, and regulates base excision repair (BER). In addition to DNA repair, USP47 is an important regulator of cell growth and genome integrity. USP47 Protein, Human (sf9, FLAG) is the recombinant human-derived USP47 protein, expressed by sf9 insect cells , with C-Flag labeled tag.
- Application
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Alternative Names
USP47; Ubiquitin carboxyl-terminal hydrolase 47; Deubiquitinating enzyme 47; Ubiquitin thioesterase 47; Ubiquitin-specific-processing protease 47
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Species
Human
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Source
Baculovirus
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Tag
C-Flag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96K76
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Expression Region
V2-D1375
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Protein Length
Partial
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Molecular Weight
158.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process











