Analytical Data
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Gene name
CNKSR3
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简介
CNKSR3 protein regulates transepithelial sodium transport by controlling aldosterone-induced ENaC-mediated sodium transport. As a scaffolding protein, CNKSR3 coordinates ENaC regulatory complex (ERC) assembly and directly interacts with ENaC subunits SCNN1A and SCNN1B. CNKSR3 Protein, Human (sf9, GST) is the recombinant human-derived CNKSR3 protein, expressed by Sf9 insect cells , with N-GST labeled tag.
- Application
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Alternative Names
Connector enhancer of kinase suppressor of ras 3; CNK3; Maguin-like protein; MAGI1
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Species
Human
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Source
Baculovirus
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Tag
N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6P9H4-1
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Expression Region
M1-V352
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Protein Length
Partial
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Molecular Weight
53-65 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CNKSR3, or Connector Enhancer of KSR3, is a member of the CNK protein family that plays a crucial role in various cellular signaling pathways, notably in the Ras-MAPK signaling cascade, which is essential for cell growth, differentiation, and survival. Research into CNKSR3 has garnered attention due to its potential implications in cancer biology, particularly as dysregulated signaling through the Ras pathway is frequently associated with tumorigenesis. Studies have shown that CNKSR3 can interact with various signaling molecules, including kinases and scaffolding proteins, suggesting that it may serve as a critical regulator of signal transduction. Additionally, CNKSR3's expression patterns in different tissues and its involvement in immune responses hint at its broader significance in physiological processes. Investigating the structure and function of CNKSR3, particularly through the production of recombinant CNKSR3 proteins, allows researchers to delineate its role in cellular signaling further. These studies could lead to insights into new therapeutic targets for diseases driven by aberrant CNKSR3 activity, including various cancers and immunological disorders. Understanding CNKSR3 at the molecular level not only helps clarify its mechanism of action but also contributes to the development of innovative strategies for intervention in related pathologies.
