Analytical Data
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Gene name
RYBP
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简介
RYBP Protein, a crucial regulatory factor, complexes with metalloproteinases like collagenases, inhibiting their activity by irreversibly binding to catalytic zinc cofactors.This interaction, notably with MMP-1, MMP-2, MMP-3, MMP-7, and MMP-9, highlights RYBP's pivotal role in finely regulating extracellular matrix remodeling and proteolytic processes across diverse physiological contexts.RYBP Protein, Human (sf9, His-GST) is the recombinant human-derived RYBP protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Application
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Alternative Names
RING1 and YY1-binding protein; APAP-1; RYBP; DEDAF; YEAF1
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Species
Human
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Source
Baculovirus
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Tag
N-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8N488
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Expression Region
M1-F228
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Protein Length
Full Length
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Molecular Weight
58 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RYBP (RING1 and YY1 Binding Protein) is a critical regulator of gene expression and chromatin dynamics, playing a significant role in various biological processes such as development, cell proliferation, and differentiation. It functions as a component of the Polycomb repressive complex 1 (PRC1), which is essential for epigenetic regulation of target genes. Research into RYBP has gained momentum due to its implications in cancer biology and genetic disorders, as altered expression or mutations of RYBP can disrupt normal cellular functions and contribute to oncogenesis. Its ability to interact with multiple protein partners, including transcription factors and chromatin modifiers, positions RYBP as a key player in coordinating complex gene regulatory networks. Understanding the structural and functional characteristics of RYBP, particularly through the study of its recombinant proteins, is crucial for elucidating its roles in chromatin remodeling and transcriptional repression. Furthermore, the development of RYBP-based therapeutic strategies has the potential to target malignant cells more effectively by restoring normal epigenetic regulation. Overall, the investigation of RYBP recombinant proteins offers valuable insights into the mechanisms underlying gene regulation and opens up avenues for novel cancer therapies.
