Analytical Data
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Gene name
SHP-1
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简介
SHP-1 Protein modulates signaling from tyrosine-phosphorylated cell surface receptors, including KIT and EGFR. It enhances the inhibition of mast cell activation via the Lilrb4a receptor. The SH2 regions interact with cellular components to regulate its phosphatase activity. In collaboration with MTUS1, it induces UBE2V2 expression upon angiotensin II stimulation. SHP-1 plays a crucial role in hematopoiesis. SHP-1 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived SHP-1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Application
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Alternative Names
Tyrosine-protein phosphatase non-receptor type 6; 70Z-SHP; PTP-1C; SHP-1; Ptpn6
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Species
Mouse
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Source
Baculovirus
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Tag
N-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P29351-2
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Expression Region
A207-K597
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Protein Length
Partial
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Molecular Weight
63 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SHP-1 (Src homology region 2 domain-containing phosphatase-1) is a protein tyrosine phosphatase that plays a critical role in the regulation of various cellular processes, including cell growth, differentiation, and immune response. It is predominantly expressed in hematopoietic cells and is known to negatively regulate signaling pathways mediated by immunoreceptors, suggesting its essential function in maintaining immune homeostasis. Dysregulation of SHP-1 has been implicated in various diseases, including autoimmune disorders and cancer, making it a significant target for therapeutic interventions. Recent studies have focused on the production of recombinant SHP-1 protein to elucidate its structural and functional properties, which could aid in understanding its role in signal transduction and disease mechanisms. By utilizing advanced techniques in recombinant DNA technology, researchers have been able to generate SHP-1 proteins that are structurally similar to their native forms, allowing for in-depth biochemical analyses and functional assays. These efforts not only enhance our understanding of SHP-1 interactions within signaling networks but also pave the way for the development of targeted therapies that can modulate its activity in pathological conditions. Overall, the study of recombinant SHP-1 protein is integral to comprehending its biological significance and potential as a therapeutic target in immune-related diseases and malignancies.
