Analytical Data
-
基因名
PON3
-
简介
PON3 is less active against organophosphates and aromatic carboxylic acid esters and is particularly capable of rapidly hydrolyzing lactones, including statin precursors such as lovastatin. It is capable of hydrolyzing aromatic lactones and specific 5- or 6-membered ring lactones, but is less efficient for simple or polar substituted lactones. PON3 Protein, Human (S50N, sf9, His) is the recombinant human-derived PON3 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
-
别名
Serum paraoxonase/lactonase 3; PON3
-
种属
Human
-
表达系统
Baculovirus
-
标签
N-His
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q15166-1
-
表达区间
M1-L354
-
蛋白长度
Full Length of Isoform-1
-
分子量
45 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PON3, or paraoxonase 3, is a member of the paraoxonase family of enzymes, which play a crucial role in lipid metabolism and antioxidant defense. Unlike its closely related homologues, PON1 and PON2, PON3 is predominantly found in the extracellular space, particularly in high-density lipoproteins (HDL). It has been implicated in various physiological processes, including the hydrolysis of toxic organophosphates and the prevention of oxidative stress, which contributes to atherogenesis and other cardiovascular diseases. Research has indicated that PON3 may have protective effects against the development of atherosclerosis and other inflammatory conditions. Given the rising prevalence of cardiovascular diseases, understanding the role of PON3 in lipid metabolism and its potential as a therapeutic target has garnered significant interest in the scientific community. Additionally, the study of PON3 recombinant proteins allows for the exploration of its enzymatic activity and interactions with various substrates, providing insights into its structural-function relationship and potential applications in medicine. Advances in biotechnology have facilitated the production and characterization of PON3, paving the way for further investigations into its role in disease and its utility in therapeutic settings. Understanding the molecular mechanisms by which PON3 operates will enhance our knowledge of cardiovascular health and may lead to new strategies for disease prevention and management.
