Analytical Data
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Gene name
HMGB3/HMG-3
- Application
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Alternative Names
HMG2A; HMG4; High mobility group protein 4
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
O15347
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Expression Region
Ser14~Ala176
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Molecular Weight
26kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of HMGB3 (High Mobility Group Box 3), also known as HMG-3, has garnered attention due to its crucial role in various cellular processes, including regulation of gene expression, DNA repair, and chromatin architecture. As a member of the high mobility group (HMG) protein family, HMGB3 possesses the ability to bind to DNA and influence its structural conformation, thus modulating the accessibility of transcription factors and other regulatory proteins. This property positions HMGB3 as a significant player in cellular responses to stress and developmental signals. Recent research has indicated that HMGB3 may be implicated in oncogenesis and the progression of certain cancers, as its expression patterns can be altered in malignant tissues. The recombinant expression of HMGB3 allows for detailed biochemical and biophysical studies, which can elucidate its functional mechanisms and interactions with DNA and other nuclear proteins. Understanding how HMGB3 operates at the molecular level could provide insights into its potential as a therapeutic target or biomarker in cancer and other diseases. Moreover, the development of recombinant HMGB3 proteins serves not only to analyze its structural and functional properties but also opens avenues for investigating its role in cellular signaling pathways, making it a focal point of study in molecular biology and medicine.
