Analytical Data
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Gene name
SHH
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简介
Sonic Hedgehog (SHH) Protein is a secretory glycoprotein of the Hedgehog family that can self-cleave to form N-SHH. SHH Protein activates Smo by binding to Frizzled and LRP6, inhibits GSK-3β to stabilize β-catenin, and activates FOXM1. SHH Protein also promotes NO release and shortens myocardial APD by phosphorylating eNOS through PI3K/Akt. SHH Protein can promote the maturation of sweat gland cells in three-dimensional culture and play a cardioprotective role in the pig ischemia-reperfusion model[1][2]. SHH Protein, Human is a recombinant human SHH protein expressed in E. coli with tag-free.
- Application
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Biological Activity
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is ≤ 0.3049 µg/mL, corresponding to a specific activity is ≥3.279×103 units/mg. Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.07853 µg/mL, corresponding to a specific activity is 1.27×104 units/mg.
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Alternative Names
Sonic Hedgehog Protein; SHH; HHG-1
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q15465
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Expression Region
C24-G197
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Protein Length
Partial
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Molecular Weight
23-25 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Sonic Hedgehog (SHH) is a crucial signaling molecule in developmental biology, primarily known for its role in the regulation of cell growth, differentiation, and patterning during embryonic development. The SHH pathway is integral for the formation of various tissues and organs, particularly the nervous system, where it influences neural stem cell behavior and neurogenesis. Abnormal SHH signaling has been implicated in various developmental disorders and diseases, including congenital malformations and certain types of cancer, such as medulloblastoma and basal cell carcinoma. Due to its significant role in both normal physiology and pathological conditions, research into SHH has focused on understanding its structure, function, and the mechanisms governing its signaling pathways. The ability to produce recombinant SHH protein has advanced these studies, allowing for detailed investigations into its biochemical properties, interaction with receptors, and downstream signaling effects. Using recombinant SHH in experimental models has enabled researchers to dissect its roles in various biological processes and to explore potential therapeutic interventions targeting SHH-related pathways. Overall, the study of SHH and its recombinant forms is vital for elucidating fundamental aspects of developmental biology and for developing strategies to address related diseases.
