Analytical Data
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Gene name
TREM-2
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简介
TREM-2 Protein is a receptor for amyloid beta protein 42, lipoprotein particles, and apolipoprotein. TREM-2 Protein is involved in cell proliferation and apoptosis through Wnt/β, MTOR, PI3K and NF-kappa-B signaling pathways. TREM-2 Protein is expressed by macrophages, immature monocyte-derived dendritic cells, osteoclasts, and microglia to activate the immune response. TREM-2 Protein, Human (HEK293, His) is the recombinant human-derived TREM-2 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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Biological Activity
Immobilized Human TREM-2, at 1 μg/mL (100 μL/well) can bind Anti-TREM2 Antibody. The ED50 is 30.5 ng/mL, corresponding to a specific activity is 3.28×10^4 units/mg. Immobilized Human TREM-2, at 1 μg/mL (100 μL/well) can bind Anti-TREM2 Antibody, The ED50 for this effect is 30.5 ng/mL, corresponding to a specific activity is 3.28×104units/mg.
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Alternative Names
Triggering receptor expressed on myeloid cells 2; TREM-2; Triggering receptor expressed on monocytes 2; TREM2
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Species
Human
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NZC2-1
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Expression Region
H19-S174
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Protein Length
Extracellular Domain
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Molecular Weight
22-40 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TREM-2 (Triggering Receptor Expressed on Myeloid Cells 2) is a receptor predominantly expressed on myeloid cells, notably microglia in the central nervous system. Its significance has surged in recent years due to its association with neurodegenerative diseases, particularly Alzheimer's disease. Genetic variants of TREM-2 have been linked to an increased risk of Alzheimer's, highlighting its role in microglial activation and immune response in the brain. Research has revealed that TREM-2 is crucial for the maintenance of microglial homeostasis and functions, influencing inflammatory responses and phagocytic activity. The interest in TREM-2 recombinant protein lies in its potential therapeutic applications. By understanding the mechanisms of TREM-2 signaling and its interactions with ligands, researchers are investigating how modulation of TREM-2 activity could enhance microglial function, potentially leading to improved outcomes in neurodegenerative conditions. Furthermore, recombinant TREM-2 protein can be used as a tool for studying its role in cellular pathways and for probing its function in immune regulation. Overall, the ongoing research into TREM-2 not only aims to elucidate its biological significance but also seeks to pave the way for innovative therapeutic strategies targeting neuroinflammation and neurodegeneration.
