Analytical Data
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基因名
Vinculin
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简介
Vinculin Protein, an actin-binding protein, is crucial for cell adhesion, morphology, and mechanosensing. It regulates E-cadherin expression and enhances mechanosensing. Vinculin forms complexes with THSD1, PTK2/FAK1, TLN1, and VCL, and interacts with APBB1IP, NRAP, CTNNB1, SYNM, SORBS1, and CTNNA1. Its interaction with CTNNA1 is necessary for localization to cell-cell junctions. Vinculin also binds to ACTN4, causing conformational changes. Vinculin Protein, Human (GST) is the recombinant human-derived Vinculin protein, expressed by E. coli , with N-GST labeled tag.
- Application
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别名
CMD1W; CMH15; Epididymis luminal protein 114; HEL114; Metavinculin; MV; MVCL; Vinculin
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种属
Human
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表达系统
E. coli
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标签
N-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P18206
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表达区间
2P-235E
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氨基酸序列
PVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVE
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蛋白长度
Partial
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Vinculin is a crucial cytoskeletal protein that plays a significant role in cell adherence, signaling, and migration by connecting integrins to the actin cytoskeleton. It is involved in various cellular processes, including mechanotransduction, which allows cells to respond to mechanical stimuli from their environment. Understanding the structure and function of vinculin is essential for elucidating its role in cellular integrity and dynamics, as well as its implications in various diseases, including cancer and heart diseases. Considering the importance of vinculin in these biological processes, researchers have focused on producing recombinant vinculin proteins to study their structure-function relationships in detail. Recombinant protein production allows for the generation of large quantities of pure protein, enabling extensive biochemical and biophysical characterization, as well as the exploration of its interactions with other cellular components. Moreover, studying recombinant vinculin can provide insights into the regulatory mechanisms of cell adhesion and motility, ultimately contributing to therapeutic strategies for diseases associated with vinculin dysfunction. Recent advancements in molecular biology techniques have facilitated the cloning, expression, and purification of vinculin, thereby enhancing its accessibility for research purposes. As researchers continue to unravel the complexities of vinculin's role in cellular processes, the development of recombinant vinculin serves as a vital tool in understanding its contributions to both normal physiology and disease pathology.
