Analytical Data
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Gene name
DCTN1
- Application
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Alternative Names
150KDA dynein-associated polypeptide DAP-150
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Species
Human
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14203
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Expression Region
213-547aa
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Molecular Weight
55.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DCTN1, or Dynactin Subunit 1, is a crucial component of the dynactin complex, which plays an essential role in cellular processes such as intracellular transport, mitosis, and the maintenance of cell shape. Mutations in the DCTN1 gene are associated with neurodegenerative diseases, particularly familial amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), highlighting its importance in neuronal function and health. Given its role in cellular dynamics, DCTN1 offers a promising target for therapeutic interventions. Research into DCTN1 recombinant proteins has gained traction as scientists aim to elucidate its structure-function relationships and regulatory mechanisms. By producing and characterizing recombinant DCTN1 proteins, researchers can gain insights into the molecular underpinnings of its interactions with motor proteins like dynein and kinesin, which are pivotal for vesicle transport along microtubules. Furthermore, understanding the biochemical properties of DCTN1 can aid in identifying pathways that lead to its dysfunction in neurodegenerative diseases. This line of investigation is not only vital for developing potential treatments but also provides a broader understanding of the fundamental aspects of cell biology and neurobiology. As such, DCTN1 recombinant protein studies are an integral part of ongoing research aimed at tackling the challenges posed by motor neuron diseases and advancing the field of neurodegeneration research.
