Analytical Data
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基因名
PNKP
- Application
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别名
DNA 5'-kinase/3'-phosphatasePolynucleotide kinase-3'-phosphatase 2 domains:Polynucleotide 3'-phosphatase (EC:3.1.3.32) ;2'(3')-polynucleotidasePolynucleotide 5'-hydroxyl-kinase (EC:2.7.1.78)
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种属
Human
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表达系统
E. coli
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标签
N- His-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96T60
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表达区间
1-521aa
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分子量
72.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PNKP (polynucleotide kinase/phosphatase) is a crucial enzyme involved in the repair of DNA and RNA, playing a significant role in maintaining cellular integrity and preventing genomic instability. Its primary functions include phosphorylating the 5' ends of nucleic acids and dephosphorylating the 3' ends, which are essential steps in various nucleic acid processing pathways, including DNA repair, recombination, and RNA processing. Mutations in the PNKP gene have been linked to neurological disorders and syndromes characterized by developmental delays, seizures, and profound sensitivity to DNA damage. Recent research has focused on developing recombinant PNKP proteins to better understand its structural and functional mechanisms, which could provide insights into therapeutic interventions for PNKP-related diseases. Such studies involve the cloning, expression, and purification of PNKP to characterize its enzymatic activities and interaction with other cellular proteins. As a result, understanding PNKP's role in nucleic acid metabolism not only sheds light on fundamental biological processes but also has significant implications for targeted gene therapy and novel treatment strategies for genetic disorders associated with DNA repair deficiencies. The continued investigation into the properties and functions of recombinant PNKP thus represents an important frontier in molecular biology and medicine.
