Analytical Data
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基因名
LacI
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简介
LacI, the lactose operon repressor, regulates lactose metabolism by binding to the operator region and repressing gene transcription. The presence of the inducer, allolactose, alters its binding affinity, derepressing the operon. As a homotetramer, LacI finely tunes lactose metabolism, orchestrating regulatory mechanisms in response to the cellular environment. LacI Protein, E.coli (His-SUMO) is the recombinant E. coli-derived LacI protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
- Application
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别名
lacI; b0345; JW0336; Lactose operon repressor
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种属
E.coli
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表达系统
E. coli
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标签
N-His;N-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P03023
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表达区间
M1-Q360
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氨基酸序列
MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
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蛋白长度
Full Length
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
LacI is a well-studied regulatory protein involved in the lactose operon of Escherichia coli, which plays a crucial role in the organism's ability to metabolize lactose. As a member of the GalR/LacI family of transcriptional regulators, LacI functions as a repressor by binding to the operator region of the lac operon, inhibiting the transcription of downstream genes responsible for lactose uptake and metabolism. The regulation of the lac operon by LacI is primarily controlled by the presence of allolactose, a metabolite of lactose that induces a conformational change in LacI, preventing its binding to DNA and allowing gene expression. Research on LacI has provided significant insights into fundamental mechanisms of gene regulation, transcriptional control, and protein-DNA interactions. Additionally, its unique binding properties and allosteric regulation have made LacI a popular model for studying protein dynamics and as a tool in synthetic biology. Investigations into LacI's structure-function relationships, including its binding affinities and conformational changes, are ongoing, as researchers aim to manipulate its activity for biotechnological applications such as biosensors and gene expression control systems. Understanding LacI's role in cellular metabolism also contributes to broader studies on bacterial adaptability and resilience, highlighting its significance in both basic and applied microbiology.
