Analytical Data
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Gene name
Exopolyphosphatase/PPX
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简介
Exopolyphosphatase/PPX protein, a key enzyme, degrades inorganic polyphosphates (polyP) by catalyzing the processive release of orthophosphate. This activity is crucial for regulating cellular polyP levels and is implicated in various biological processes. The protein's role in polyP degradation contributes to maintaining phosphate homeostasis and is integral to the intricate network of cellular processes involving polyP. Exopolyphosphatase/PPX Protein, E.coli (His) is the recombinant E. coli-derived Exopolyphosphatase/PPX protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
ppx; c3020; Exopolyphosphatase; ExopolyPase; EC 3.6.1.11
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Species
E.coli
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0AFL7
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Expression Region
P2-A513
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AA Sequence
PIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQRVHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFLKRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPILVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASGTIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLAILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQARRVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNSDLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEESTPEIAA
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Protein Length
Full Length of Mature Protein
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Molecular Weight
62.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Exopolyphosphatase (PPX) is an important enzyme involved in the hydrolysis of polyphosphate chains into inorganic phosphate, playing a critical role in various biological processes, including energy metabolism and stress responses in microorganisms. The study of recombinant PPX proteins has garnered significant interest due to their potential applications in biotechnology and medicine, particularly in bioremediation, where polyphosphate metabolism can influence nutrient cycling and microbial community dynamics. The characterization of PPX at the molecular and structural levels aids in understanding its enzymatic mechanism and regulation, providing insights into its role in cellular physiology. Moreover, recombinant expression of PPX allows for the production of large quantities of the enzyme, enabling detailed biochemical studies and the exploration of its applications, such as in the development of novel phosphate recovery strategies and sustainable agricultural practices. Ongoing research aims to optimize the properties of recombinant PPX through mutagenesis and engineering approaches, ultimately enhancing its functionality for practical applications. This background underscores the significance of exopolyphosphatase in both fundamental biological research and its potential in addressing real-world challenges related to phosphorus management and environmental sustainability.
