Analytical Data
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Gene name
TMED1
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简介
The TMED1 protein may facilitate vesicular protein trafficking in the early secretory pathway, acting as a cargo receptor on the luminal side to incorporate secretory cargo into transport vesicles. It is involved in the formation of the vesicle shell on the cytoplasmic side and positively affects IL-33-mediated IL-8 and IL-6 production by interacting with IL1RL1. TMED1 Protein, Human (HEK293, His) is the recombinant human-derived TMED1 protein, expressed by HEK293 , with C-10*His labeled tag.
- Application
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Biological Activity
Immobilized Human TMED1 at 10 μg/mL (100 μL/well) can bind Biotinylated ST2 protein. The ED50 for this effect is 79.64 ng/mL, corresponding to a specific activity is 1.26×10^4 Unit/mg. Immobilized Human TMED1 at 10 μg/mL (100 μL/well) can bind Biotinylated ST2 protein. The ED50 for this effect is 79.64 ng/mL, corresponding to a specific activity is 1.26×104 Unit/mg.
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Alternative Names
Transmembrane emp24 domain-containing protein 1; Interleukin-1 receptor-like 1 ligand; Tp24; IL1RL1L; IL1RL1LG
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Species
Human
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Source
HEK293
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Tag
C-10*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q13445
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Expression Region
A24-N194
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Protein Length
Extracellular Domain
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Molecular Weight
28 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TMED1, a member of the transmembrane emp24 domain (TMED) family, plays a crucial role in the transport of proteins within the cell, particularly in the endoplasmic reticulum (ER) and the Golgi apparatus. Research into TMED1 has gained momentum due to its involvement in various cellular processes, including protein sorting, secretion, and the modulation of ER stress responses. Dysregulation of TMED1 has been implicated in several diseases, such as cancer and neurodegenerative disorders, making it a potential target for therapeutic interventions. Understanding the structure and function of TMED1 at a molecular level is essential for elucidating its role in these pathological conditions. Recombinant TMED1 proteins are being produced to study their biochemical properties, interactome, and functional dynamics in cellular contexts. This research is expected to shed light on the mechanistic pathways that TMED1 influences, contributing to the identification of novel biomarkers or drug targets for diseases associated with protein misfolding and trafficking. Overall, the study of TMED1 not only enhances our understanding of protein transport mechanisms but also opens avenues for potential clinical applications in treating TMED1-related disorders.
