Analytical Data
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Gene name
Semaphorin-4G/SEMA4G
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简介
SEMA4G Protein, a cell surface receptor for PLXNB2, potentially participates in axon guidance. It forms an interaction with PLXNB2. Semaphorin-4G/SEMA4G Protein, Human (HEK293, His) is the recombinant human-derived Semaphorin-4G/SEMA4G protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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Alternative Names
Semaphorin-4G; KIAA1619; SEMA4G
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Species
Human
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NTN9-2
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Expression Region
V18-L680
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AA Sequence
VPGPSLRRPSRELDATPRMTIPYEELSGTRHFKGQAQNYSTLLLEEASARLLVGARGALFSLSANDIGDGAHKEIHWEASPEMQSKCHQKGKNNQTECFNHVRFLQRLNSTHLYACGTHAFQPLCAAIDAEAFTLPTSFEEGKEKCPYDPARGFTGLIIDGGLYTATRYEFRSIPDIRRSRHPHSLRTEETPMHWLNDAEFVFSVLVRESKASAVGDDDKVYYFFTERATEEGSGSFTQSRSSHRVARVARVCKGDLGGKKILQKKWTSFLKARLICHIPLYETLRGVCSLDAETSSRTHFYAAFTLSTQWKTLEASAICRYDLAEIQAVFAGPYMEYQDGSRRWGRYEGGVPEPRPGSCITDSLRSQGYNSSQDLPSLVLDFVKLHPLMARPVVPTRGRPLLLKRNIRYTHLTGTPVTTPAGPTYDLLFLGTADGWIHKAVVLGSGMHIIEETQVFRESQSVENLVISLLQHSLYVGAPSGVIQLPLSSCSRYRSCYDCILARDPYCGWDPGTHACAAATTIANRSQGSRTALIQDIERGNRGCESSRDTGPPPPLKTRSVLRGDDVLLPCDQPSNLARALWLLNGSMGLSDGQGGYRVGVDGLLVTDAQPEHSGNYGCYAEENGLRTLLASYSLTVRPATPAPAPKAPATPGAQLAPDVRL
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Protein Length
Partial
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Molecular Weight
90-95 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Semaphorin-4G (SEMA4G) is a member of the semaphorin family, which is known for its roles in various biological processes, including axon guidance, immune responses, and tumor progression. This specific semaphorin functions as a signaling molecule that interacts with neuropilin and plexin receptors, influencing cellular behaviors such as migration, proliferation, and differentiation. Research has highlighted SEMA4G's involvement in the immune system, particularly in modulating T cell responses, where it can act as an immune checkpoint molecule. Furthermore, its expression has been linked to tumorigenesis, raising interest in SEMA4G as a potential therapeutic target in cancer treatment. The generation of recombinant SEMA4G proteins allows for in-depth studies of its structural and functional properties, enabling researchers to elucidate its mechanisms of action and its role in pathophysiological contexts. Understanding the dynamics of SEMA4G signaling pathways could lead to novel interventions in diseases where these pathways are dysregulated, thereby contributing to advancements in immunotherapy and targeted cancer treatments. Overall, the study of SEMA4G is a promising area of research with significant implications for both fundamental biology and clinical applications.
