Analytical Data
-
Gene name
ADI
-
简介
ADI Proteinas, central to the ADI pathway, catalyzes the initial step in L-arginine degradation. Specifically, it converts L-arginine to carbamoyl phosphate, initiating the pathway and playing a key role in regulating the overall metabolic breakdown of L-arginine. ADI Protein, Metamycoplasma hominis (HEK293, His) is the recombinant ADI protein, expressed by HEK293 , with C-10*His labeled tag.
- Application
-
Alternative Names
Arginine deiminase; Arginine dihydrolase (AD); arcA
-
Species
Others
-
Source
HEK293
-
Tag
C-10*His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P41141
-
Expression Region
S2-W409
-
AA Sequence
SVFDSKFNGIHVYSEIGELETVLVHEPGREIDYITPARLDELLFSAILESHDARKEHQSFVKIMKDRGINVVELTDLVAETYDLASKAAKEEFIETFLEETVPVLTEANKKAVRAFLLSKPTHEMVEFMMSGITKYELGVESENELIVDPMPNLYFTRDPFASVGNGVTIHFMRYIVRRRETLFARFVFRNHPKLVKTPWYYDPAMKMPIEGGDVFIYNNETLVVGVSERTDLDTITLLAKNIKANKEVEFKRIVAINVPKWTNLMHLDTWLTMLDKNKFLYSPIANDVFKFWDYDLVNGGAEPQPQLNGLPLDKLLASIINKEPVLIPIGGAGATEMEIARETNFDGTNYLAIKPGLVIGYDRNEKTNAALKAAGITVLPFHGNQLSLGMGNARCMSMPLSRKDVKW
-
Protein Length
Full Length of Mature Protein
-
Molecular Weight
50kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of ADI (arginine deiminase) recombinant proteins has garnered significant attention due to their potential applications in cancer therapy and microbial pathogenesis. Arginine deiminase is an enzyme that catalyzes the conversion of arginine to citrulline and ammonia, and it has been identified as a promising therapeutic target, especially in arginine-restricted tumors, where certain cancer cells are unable to synthesize arginine and rely on external sources. By exploiting the depletion of arginine, ADI can induce cell death in these cancer cells, making it a compelling candidate for targeted treatments. Additionally, ADI has been investigated in the context of various pathogenic microorganisms, where it plays a role in the survival of these pathogens in arginine-limited environments, such as during host immune responses. The ability to produce recombinant ADI proteins allows researchers to study their structure and function in detail, facilitating the development of novel therapeutic strategies and the understanding of microbial adaptation mechanisms. Furthermore, the optimization of ADI for therapeutic uses involves recombinant expression systems, purification methods, and functional assays, positioning ADI as a critical focus in biotechnology and medical research. This multi-faceted interest in ADI recombinant proteins reflects their potential to provide innovative solutions in the realm of cancer treatment and infectious disease management, making them pivotal subjects in contemporary biochemical and pharmaceutical studies.
