Analytical Data
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基因名
ADI
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简介
ADI Proteinas, central to the ADI pathway, catalyzes the initial step in L-arginine degradation. Specifically, it converts L-arginine to carbamoyl phosphate, initiating the pathway and playing a key role in regulating the overall metabolic breakdown of L-arginine. ADI Protein, Metamycoplasma hominis (HEK293, His) is the recombinant ADI protein, expressed by HEK293 , with C-10*His labeled tag.
- Application
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别名
Arginine deiminase; Arginine dihydrolase (AD); arcA
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种属
Others
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表达系统
HEK293
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标签
C-10*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P41141
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表达区间
S2-W409
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氨基酸序列
SVFDSKFNGIHVYSEIGELETVLVHEPGREIDYITPARLDELLFSAILESHDARKEHQSFVKIMKDRGINVVELTDLVAETYDLASKAAKEEFIETFLEETVPVLTEANKKAVRAFLLSKPTHEMVEFMMSGITKYELGVESENELIVDPMPNLYFTRDPFASVGNGVTIHFMRYIVRRRETLFARFVFRNHPKLVKTPWYYDPAMKMPIEGGDVFIYNNETLVVGVSERTDLDTITLLAKNIKANKEVEFKRIVAINVPKWTNLMHLDTWLTMLDKNKFLYSPIANDVFKFWDYDLVNGGAEPQPQLNGLPLDKLLASIINKEPVLIPIGGAGATEMEIARETNFDGTNYLAIKPGLVIGYDRNEKTNAALKAAGITVLPFHGNQLSLGMGNARCMSMPLSRKDVKW
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蛋白长度
Full Length of Mature Protein
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分子量
50kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of ADI (arginine deiminase) recombinant proteins has garnered significant attention due to their potential applications in cancer therapy and microbial pathogenesis. Arginine deiminase is an enzyme that catalyzes the conversion of arginine to citrulline and ammonia, and it has been identified as a promising therapeutic target, especially in arginine-restricted tumors, where certain cancer cells are unable to synthesize arginine and rely on external sources. By exploiting the depletion of arginine, ADI can induce cell death in these cancer cells, making it a compelling candidate for targeted treatments. Additionally, ADI has been investigated in the context of various pathogenic microorganisms, where it plays a role in the survival of these pathogens in arginine-limited environments, such as during host immune responses. The ability to produce recombinant ADI proteins allows researchers to study their structure and function in detail, facilitating the development of novel therapeutic strategies and the understanding of microbial adaptation mechanisms. Furthermore, the optimization of ADI for therapeutic uses involves recombinant expression systems, purification methods, and functional assays, positioning ADI as a critical focus in biotechnology and medical research. This multi-faceted interest in ADI recombinant proteins reflects their potential to provide innovative solutions in the realm of cancer treatment and infectious disease management, making them pivotal subjects in contemporary biochemical and pharmaceutical studies.
