Analytical Data
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Gene name
TrpB
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简介
The TrpB protein, especially its β subunit, plays a crucial role in the synthesis of L-tryptophan from indole and L-serine. This enzyme function involves converting precursor molecules into the essential amino acid L-tryptophan, thereby facilitating its biosynthetic pathway. TrpB Protein, E.coli (His) is the recombinant E. coli-derived TrpB protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
Tryptophan synthase beta chain; trpB
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Species
E.coli
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0A879
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Expression Region
T2-I397
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AA Sequence
TTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQFNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMGKTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVHSGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILEREGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRVGIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALEAFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILKARGEI
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Protein Length
Full Length of Mature Protein
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Molecular Weight
38-48 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TrpB, also known as tryptophan synthase beta subunit, is a crucial enzyme in the biosynthesis of the essential amino acid tryptophan, which plays a vital role in protein synthesis and serves as a precursor for various bioactive compounds, including neurotransmitters like serotonin. The study of TrpB recombinant proteins has gained significant attention due to their potential applications in biotechnology and therapeutic development. Recombinant expression systems allow for the production of TrpB in large quantities, facilitating detailed biochemical and structural studies that can uncover its catalytic mechanisms and interactions with various substrates. Additionally, understanding TrpB's structure-function relationship can aid in the design of inhibitors that may serve as novel antimicrobial agents, given the enzyme’s presence in many pathogenic bacteria. The manipulation of TrpB through recombinant techniques not only enhances our understanding of tryptophan biosynthesis but also opens doors for the engineering of metabolic pathways in microorganisms for improved production of tryptophan and related metabolites. With growing interest in metabolic engineering and synthetic biology, TrpB becomes a key target for research aimed at developing sustainable bioprocesses, making it an intensively studied protein in the fields of enzyme catalysis, metabolic engineering, and pharmaceutical development.
