Analytical Data
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Gene name
IL-32
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简介
IL-32 protein is a member of the cytokine family whose expression increases following T cell activation or IL-2-induced NK cell activation. It plays a key role in inducing macrophages to produce TNFα, which contributes to the inflammatory response. IL-32 alphaProtein, Human (HEK293, His) is the recombinant human-derived IL-32 alpha protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 0.5-3 μg/mL in the presence of 5 μg/mL MDP. Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 1.398 μg/mL in the presence of 5μg/mL MDP. Corresponding to a specific activity is 715.308 U/mg.
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Alternative Names
Interleukin-32; IL-32; NK4; TAIF; IL-32 alpha
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Species
Human
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 95% as determined by reducing SDS-PAGE. as determined by SDS-PAGE.
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Uniprot
P24001
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Expression Region
M1-K131
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Protein Length
Full Length of Isoform-4
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Molecular Weight
20 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
IL-32, or Interleukin-32, is a pro-inflammatory cytokine that plays a significant role in various immune responses and inflammatory diseases. Discovered in 2005, IL-32 is primarily produced by activated immune cells such as T cells, monocytes, and macrophages in response to infections and inflammatory stimuli. Its diverse biological functions include the regulation of immune cell activation, induction of other pro-inflammatory cytokines, and modulation of apoptosis in infected or cancerous cells. The elucidation of IL-32's mechanisms has significant implications for understanding its role in diseases like rheumatoid arthritis, inflammatory bowel disease, and certain cancers. Consequently, research into IL-32 recombinant proteins has gained momentum, aiming to explore their therapeutic potential. By producing and studying IL-32 recombinant proteins, researchers aim to develop targeted therapies that can modulate inflammatory responses, offering new avenues for the treatment of chronic inflammatory conditions. Additionally, understanding the structure-function relationship of IL-32 could pave the way for designing novel immunotherapies, enhancing the efficacy of existing treatments, and minimizing side effects. Overall, IL-32 remains an intriguing focus within immunological research, with promising prospects for biomedical applications.
