Analytical Data
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基因名
EIF5A2
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简介
EIF5A2 is a translation factor critical for promoting elongation and termination, helping stalled ribosomes encounter specific amino acid environments. EIF5A2 is located between the ribosome exit (E) and peptidyl (P) sites and rescues stalled ribosomes, especially those translating polyproline-containing peptides. EIF5A2 Protein, Human (His) is the recombinant human-derived EIF5A2 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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别名
Eukaryotic translation initiation factor 5A-2; Eukaryotic initiation factor 5A isoform 2; EIF5A2
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9GZV4
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表达区间
M1-K153
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蛋白长度
Full Length
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分子量
22 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EIF5A2, or Eukaryotic Translation Initiation Factor 5A2, is a crucial protein involved in the regulation of translation and cellular stress responses. Research on EIF5A2 has gained momentum due to its significant role in various cellular processes, including cell proliferation, differentiation, and apoptosis. This protein is unique in containing a modified amino acid, hypusine, which is essential for its function in translation initiation and elongation. Aberrant expression of EIF5A2 has been implicated in several cancers, highlighting its potential as a therapeutic target and a biomarker for tumor progression. In recent studies, the recombinant expression of EIF5A2 has been utilized to investigate its functional properties and interaction with other cellular components, paving the way for understanding the molecular mechanisms underlying its role in health and disease. Furthermore, the study of EIF5A2 in the context of stress conditions, such as hypoxia and nutrient deprivation, has revealed its adaptive functions that are critical for cellular survival. Given these findings, ongoing research aims to elucidate the precise mechanisms by which EIF5A2 contributes to oncogenesis and its potential utility in clinical applications, including cancer diagnosis and therapy.
