Analytical Data
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基因名
ATG4A
- Application
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别名
rHuATG4A, His; Cysteine Protease ATG4A; AUT-Like 2 Cysteine Endopeptidase; ATG4A; APG4A; AUTL2
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q8WYN0-1
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表达区间
M1-V398
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氨基酸序列
MESVLSKYEDQITIFTDYLEEYPDTDELVWILGKQHLLKTEKSKLLSDISARLWFTYRRKFSPIGGTGPSSDAGWGCMLRCGQMMLAQALICRHLGRDWSWEKQKEQPKEYQRILQCFLDRKDCCYSIHQMAQMGVGEGKSIGEWFGPNTVAQVLKKLALFDEWNSLAVYVSMDNTVVIEDIKKMCRVLPLSADTAGDRPPDSLTASNQSKGTSAYCSAWKPLLLIVPLRLGINQINPVYVDAFKECFKMPQSLGALGGKPNNAYYFIGFLGDELIFLDPHTTQTFVDTEENGTVNDQTFHCLQSPQRMNILNLDPSVALGFFCKEEKDFDNWCSLVQKEILKENLRMFELVQKHPSHWPPFVPPAKPEVTTTGAEFIDSTEQLEEFDLEEDFEILSV
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蛋白长度
Full Length of Isoform-1
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分子量
40-55 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ATG4A, or autophagy-related protein 4A, plays a crucial role in the autophagy process, a cellular mechanism essential for degrading and recycling cellular components. This protein is part of the ATG4 family, which is characterized by their cysteine protease activity involved in the processing of autophagy proteins. Research has shown that ATG4A is essential for the cleavage of the LC3 precursor protein, converting it into its lipidated form, LC3-II, which is critical for the formation of autophagosomes. Dysregulation of autophagy and, by extension, ATG4A is linked to various pathologies, including neurodegenerative diseases, cancer, and infections. Scientists are increasingly investigating the activity and regulation of ATG4A to better understand its functions and implications in these diseases, as well as its potential as a therapeutic target. Recombinant ATG4A protein studies provide insights into its enzymatic properties, interaction with other proteins, and effects on cellular autophagic processes. This research is significant for developing new strategies to manipulate autophagy for therapeutic benefits, particularly in diseases where autophagy is impaired or altered.
