Analytical Data
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基因名
MICB
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简介
MICB Protein, a heavily glycosylated ligand for NKG2D receptor, triggers cytolytic response in immune cells. It resembles MHC class I molecules but lacks association with beta-2-microglobulin or peptide binding. Alternative splicing leads to multiple transcript variants. MICB is widely expressed, especially in lymph nodes and bone marrow, highlighting its immune response role in diverse physiological contexts. MICB Protein, Human (HEK293, His) is the recombinant human-derived MICB protein, expressed by HEK293 , with C-His, C-6*His labeled tag.
- Application
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生物活性
Immobilized Human NKG2D/CD314 (HY-P76512) at 2 μg/mL (100 μL/well) can bind Human MICB. The ED50 for this effect is 115.3 ng/mL. Immobilized Human NKG2D/CD314 (HY-P76512) at 2 μg/mL (100 μL/well) can bind Human MICB. The ED50 for this effect is 115.3 ng/mL.
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别名
MHC class I polypeptide-related sequence B; MIC-B; PERB11.2
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
NP_005922.2
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表达区间
A23-G298
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氨基酸序列
AEPHSLRYNLMVLSQDGSVQSGFLAEGHLDGQPFLRYDRQKRRAKPQGQWAENVLGAKTWDTETEDLTENGQDLRRTLTHIKDQKGGLHSLQEIRVCEIHEDSSTRGSRHFYYDGELFLSQNLETQESTVPQSSRAQTLAMNVTNFWKEDAMKTKTHYRAMQADCLQKLQRYLKSGVAIRRTVPPMVNVTCSEVSEGNITVTCRASSFYPRNITLTWRQDGVSLSHNTQQWGDVLPDGNGTYQTWVATRIRQGEEQRFTCYMEHSGNHGTHPVPSG
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蛋白长度
Partial
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分子量
45 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MICB (MHC class I polypeptide-related sequence B) is a key molecule implicated in the immune response against malignant cells and viral infections. It is a member of the natural killer (NK) cell receptor ligand family and plays a critical role in the recognition and elimination of stressed or transformed cells by NK cells. Understanding MICB's structure and function is essential for developing novel immunotherapeutic strategies, particularly in cancer treatment. Recent studies have shown that MICB expression can be upregulated in various tumors, making it a potential target for immunotherapy. Research has focused on the generation of recombinant MICB proteins to elucidate their role in immune modulation, assess their interaction with NK cell receptors, and evaluate their efficacy in adopting strategies like CAR-NK cell therapy. Moreover, recombinant MICB proteins can serve as valuable tools for studying the molecular mechanisms that govern NK cell activation and tolerance, thereby advancing our understanding of immune responses in tumor microenvironments. As a result, the study of MICB recombinant proteins holds promise for improving therapeutic outcomes in oncology and infectious disease management.
