Analytical Data
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基因名
PHPT1
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简介
The PHPT1 protein acts as a phosphohistidine phosphatase, catalyzing the dephosphorylation of phosphohistidine residues. This unique enzymatic activity makes PHPT1 a regulator of histidine phosphorylation events, suggesting that it is involved in signaling pathways or cellular processes that are critical for phosphohistidine modification. PHPT1 Protein, Human (His) is the recombinant human-derived PHPT1 protein, expressed by E. coli , with N-His labeled tag.
- Application
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生物活性
Measured in a cell proliferation assay using A549 cells. The ED50 for this effect is 43.24 ng/mL. Measured in a cell proliferation assay using A549 cells. The ED50 for this effect is 43.24 ng/mL, corresponding to a specific activity is 2.31×104 units/mg.
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别名
14 kDa phosphohistidine phosphatase; PHPT1; PHP; PHP14; CGI-202; HSPC141
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NRX4-1
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表达区间
A2-Y125
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蛋白长度
Full Length of Isoform-1 Mature Protein
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分子量
15.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ST2, also known as IL1RL1, is a receptor for interleukin-33 (IL-33), a member of the IL-1 cytokine family. This protein is crucial for mediating immune responses, particularly in the context of inflammation and allergic diseases. Elevated levels of ST2 and IL-33 have been associated with various pathological conditions, including asthma, cardiovascular diseases, and fibrosis. Understanding the biology of ST2 can provide insights into its role in immune regulation and disease pathogenesis. Recent studies have focused on the development of recombinant ST2 proteins to further explore its functions and potential therapeutic applications. These recombinant proteins can be utilized in assays to investigate the receptor's signaling pathways, interactions with other cytokines, and its role in modulating immune responses. Moreover, recombinant ST2 has emerged as a candidate for biomarker development, given its association with disease severity in asthma and heart failure. Research on this protein is paving the way for novel therapeutic strategies aimed at targeting the ST2/IL-33 axis, with the potential to alleviate inflammatory diseases and improve patient outcomes.
