Analytical Data
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Gene name
PHPT1
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简介
The PHPT1 protein acts as a phosphohistidine phosphatase, catalyzing the dephosphorylation of phosphohistidine residues. This unique enzymatic activity makes PHPT1 a regulator of histidine phosphorylation events, suggesting that it is involved in signaling pathways or cellular processes that are critical for phosphohistidine modification. PHPT1 Protein, Human (His) is the recombinant human-derived PHPT1 protein, expressed by E. coli , with N-His labeled tag.
- Application
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Biological Activity
Measured in a cell proliferation assay using A549 cells. The ED50 for this effect is 43.24 ng/mL. Measured in a cell proliferation assay using A549 cells. The ED50 for this effect is 43.24 ng/mL, corresponding to a specific activity is 2.31×104 units/mg.
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Alternative Names
14 kDa phosphohistidine phosphatase; PHPT1; PHP; PHP14; CGI-202; HSPC141
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NRX4-1
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Expression Region
A2-Y125
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Protein Length
Full Length of Isoform-1 Mature Protein
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Molecular Weight
15.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ST2, also known as IL1RL1, is a receptor for interleukin-33 (IL-33), a member of the IL-1 cytokine family. This protein is crucial for mediating immune responses, particularly in the context of inflammation and allergic diseases. Elevated levels of ST2 and IL-33 have been associated with various pathological conditions, including asthma, cardiovascular diseases, and fibrosis. Understanding the biology of ST2 can provide insights into its role in immune regulation and disease pathogenesis. Recent studies have focused on the development of recombinant ST2 proteins to further explore its functions and potential therapeutic applications. These recombinant proteins can be utilized in assays to investigate the receptor's signaling pathways, interactions with other cytokines, and its role in modulating immune responses. Moreover, recombinant ST2 has emerged as a candidate for biomarker development, given its association with disease severity in asthma and heart failure. Research on this protein is paving the way for novel therapeutic strategies aimed at targeting the ST2/IL-33 axis, with the potential to alleviate inflammatory diseases and improve patient outcomes.
