Recombinant Human IL-23R Protein (HEK293), C-hFc

IL-23R

IL-23R Protein, critical in immune response, forms the interleukin-23 receptor by associating with IL12RB1. Binding to IL23, it activates T-cells, NK cells, and potentially macrophage/myeloid cells through Jak-Stat signaling. Associated with autoimmune diseases and tumorigenesis, IL-23R, in the presence of IL12RB1, forms a heterodimeric IL-23 receptor, interacting with JAK2 and activating STAT3. IL-23R Protein, Human (HEK293, Fc) is the recombinant human-derived IL-23R protein, expressed by HEK293 , with C-hFc labeled tag.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

1. Measured by its binding ability in a functional ELISA. Immobilized human IL23A-His+IL12B-His at 10 μg/mL (100 μl/well) can bind human IL23R-Fc and the EC50 is 0.28-0.66 μg/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized mouse IL12Bh (m)+mIL23Ah at 10 μg/mL (100 μl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.14-0.35 μg/mL. 3.Measured in a cell proliferation assay using M-NFS-60 cells. The ED50 this effect is 0.9517 μg/mL, corresponding to a specific activity is 1.05×103 units/mg in the presence of 0.125 ng/mL of recombinant human G-CSF. 4. Measured by its ability to inhibit IL-23 induced IL-17 secretion by Jurkat cells. The ED50 for this effect is 0.03289 μg/mL in the presence of 1 ng/mL IL-23, corresponding to a specific activity is 3.04×104 U/mg.

Interleukin 23 receptor; IL 23R; IL-23 receptor; IL-23R

Human

HEK293

C-hFc

Greater than 90% as determined by reducing SDS-PAGE as determined by SDS-PAGE.

Q5VWK5

G24-D353

GITNINCSGHIWVEPATIFKMGMNISIYCQAAIKNCQPRKLHFYKNGIKERFQITRINKTTARLWYKNFLEPHASMYCTAECPKHFQETLICGKDISSGYPPDIPDEVTCVIYEYSGNMTCTWNAGKLTYIDTKYVVHVKSLETEEEQQYLTSSYINISTDSLQGGKKYLVWVQAANALGMEESKQLQIHLDDIVIPSAAVISRAETINATVPKTIIYWDSQTTIEKVSCEMRYKATTNQTWNVKEFDTNFTYVQQSEFYLEPNIKYVFQVRCQETGKRYWQPWSSLFFHKTPETVPQVTSKAFQHDTWNSGLTVASISTGHLTSDNRGD

Extracellular Domain

90-110 kDa

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.