Analytical Data
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Gene name
Carbonic Anhydrase 9
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简介
Carbonic Anhydrase 9 Protein catalyzes the reversible hydration of carbon dioxide, essential for converting it to bicarbonate ions and protons, and vice versa. This enzymatic activity is crucial in physiological processes like acid-base balance regulation, respiration, and cellular pH homeostasis. Carbonic Anhydrase 9 Protein is vital for carbon dioxide transport and metabolism, contributing to overall regulation of its levels in the body. Carbonic Anhydrase 9 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Carbonic Anhydrase 9 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Alternative Names
CA9; CAIX; CA-IX; EC 4.2.1.1; G250; MN; P54/58N; PMW1; RCC
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Species
Cynomolgus
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Source
HEK293
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Tag
C-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A0A2K5VQG9
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Expression Region
Q38-D402
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Protein Length
Extracellular Domain
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Molecular Weight
50-60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Carbonic Anhydrase 9 (CA9) is a prominent member of the carbonic anhydrase enzyme family, which plays a critical role in regulating carbon dioxide and bicarbonate levels in various physiological processes. CA9 is particularly expressed in hypoxic tumors and is associated with tumor growth and metastasis, making it a potential biomarker for cancer diagnosis and a target for therapeutic intervention. Overexpression of CA9 in malignant tissues often correlates with poor prognosis, highlighting its significance in cancer biology. The recombinant form of CA9 is essential for studying its biochemical properties, understanding its catalytic mechanism, and evaluating its role in tumor microenvironments. Additionally, the production of recombinant CA9 enables the development of specific inhibitors or antibodies that can aid in cancer treatment strategies. Research involving CA9 is further motivated by its potential in targeted therapies, as blocking its activity may disrupt tumor acidic microenvironments and enhance the efficacy of conventional treatments. Overall, the study of CA9 and its recombinant protein form is crucial for advancing our understanding of cancer biology and developing novel therapeutic approaches.
