Analytical Data
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Gene name
IL-21
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简介
IL-21 is a potent immunoregulatory cytokine that orchestrates the delicate interplay between innate and adaptive immunity. It drives the production of IgG(1) and IgG(3) in B cells, which are critical for humoral immune responses, and plays a key role in T follicular helper (Tfh) cell generation and germinal center formation. IL-21 Protein, Canine is the recombinant canine-derived IL-21 protein, expressed by E. coli , with tag free.
- Application
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Biological Activity
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells.The ED50 for this effect is typically 0.4-7.767 ng/mL. Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is 7.767 ng/mL, corresponding to a specific activity is 1.287×105 U/mg.
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Alternative Names
Interleukin-21; Il21; Za11
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Species
Canine
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by reducing SDS-PAGE. as determined by SDS-PAGE.
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Uniprot
Q6L7I9
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Expression Region
H18-S146
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Protein Length
Full Length of Mature Protein
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Molecular Weight
15 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Interleukin-21 (IL-21) is a crucial cytokine involved in the regulation of immune responses, primarily produced by T-follicular helper cells, natural killer cells, and certain subsets of CD4+ T cells. It plays a significant role in the differentiation and activation of B cells, promoting antibody production, and modulating the functions of various immune cells, including T cells and dendritic cells. Due to its vital function in both adaptive and innate immunity, IL-21 has garnered substantial interest in therapeutics, particularly in the context of autoimmune diseases, cancer, and viral infections. The recombinant IL-21 protein has been developed to explore its potential clinical applications, providing a tool for understanding its mechanisms in immune modulation. Research indicates that IL-21 can enhance anti-tumor immunity and improve vaccine responses, making it a promising candidate for immunotherapy. Moreover, studies have suggested that modulating IL-21 activity could have therapeutic benefits in conditions characterized by immune dysregulation, such as systemic lupus erythematosus and rheumatoid arthritis. Ongoing investigations aim to elucidate its precise roles and optimize its application in clinical settings, including combination therapies that leverage IL-21's ability to boost immune responses against malignancies or chronic infections. Overall, the exploration of IL-21 recombinant protein serves as a pivotal step toward harnessing its immunomodulatory properties for innovative treatment strategies in various diseases.
