Analytical Data
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基因名
Glypican-3/GPC3
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简介
The Glypican-3 (GPC3) protein is a cell surface proteoglycan that plays complex regulatory roles in key signaling pathways critical to developmental processes. Through its GPI anchoring, GPC3 negatively regulates the Hedgehog signaling pathway by competing with the Hedgehog receptor PTC1 for binding to Hedgehog protein, leading to complex internalization and subsequent lysosomal degradation. Glypican-3/GPC3 Protein, Human (HEK293, Llama Fc) is the recombinant human-derived Glypican-3/GPC3 protein, expressed by HEK293, with C-Llama Fc labeled tag.
- Application
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别名
rHuGlypican-3/GPC3, Fc; Glypican-3; GTR2-2; Intestinal protein OCI-5; MXR7; GPC3; OCI5
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种属
Human
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表达系统
HEK293
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标签
C-Llama Fc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P51654-1
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表达区间
Q25-H559
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蛋白长度
Partial
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分子量
40 kDa & 60 kDa & 100-120 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Glypican-3 (GPC3) is a heparan sulfate proteoglycan that plays a crucial role in cell growth and development, particularly in the context of various cancers. It is predominantly expressed in fetal tissues and is typically downregulated in normal adult tissues, making it a significant marker for certain tumors, especially hepatocellular carcinoma (HCC) and Wilms' tumor. The aberrant expression of GPC3 in tumors is associated with poor clinical outcomes, highlighting its potential as a therapeutic target and a diagnostic biomarker. Research into GPC3 has intensified due to its unique characteristics; it modulates signaling pathways such as Wnt, Hedgehog, and insulin-like growth factor, which are crucial for cell proliferation and differentiation. As a result, recombinant GPC3 protein has been developed for various applications, including targeted therapies and vaccine development against GPC3-expressing tumors. The study of GPC3 also opens avenues for understanding its role in tumor microenvironments and interactions with other cellular receptors, aiming to harness its properties for developing novel cancer treatments. Given its pivotal role in oncogenesis and tumor progression, GPC3 remains a focal point of ongoing research efforts in the fields of oncology and regenerative medicine.
