Analytical Data
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基因名
L-selectin/CD62L
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简介
The L-selectin/CD62L protein is a calcium-dependent lectin that promotes cell adhesion by binding to glycoproteins on neighboring cells. L-selectin/CD62L Protein, Human (HEK293, His) is the recombinant human-derived L-selectin/CD62L protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured by the ability of the immobilized protein to support the adhesion of THP-1 human prostate cancer cells. The ED50 for this effect is 1.0-3.0 μg/mL. Measured by the ability of the immobilized protein to support the adhesion of THP-1 human prostate cancer cells. The ED50 for this effect is 2.231 μg/mL, corresponding to a specific activity is 448.230 units/mg.
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别名
L-selectin; Sell; CD62 antigen-like family member L; LECAM1; CD62L; LAM-1
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P14151
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表达区间
W39-N332
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氨基酸序列
WTYHYSEKPMNWQRARRFCRDNYTDLVAIQNKAEIEYLEKTLPFSRSYYWIGIRKIGGIWTWVGTNKSLTEEAENWGDGEPNNKKNKEDCVEIYIKRNKDAGKWNDDACHKLKAALCYTASCQPWSCSGHGECVEIINNYTCNCDVGYYGPQCQFVIQCEPLEAPELGTMDCTHPLGNFSFSSQCAFSCSEGTNLTGIEETTCGPFGNWSSPEPTCQVIQCEPLSAPDLGIMNCSHPLASFSFTSACTFICSEGTELIGKKKTICESSGIWSNPSPICQKLDKSFSMIKEGDYN
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蛋白长度
Extracellular Domain
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分子量
50-75 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
L-selectin, also known as CD62L, is a cell adhesion molecule primarily expressed on leukocytes, playing a critical role in the recruitment and homing of immune cells to sites of inflammation and lymphoid organs. Its function is mediated through interactions with specific carbohydrates expressed on endothelial cells and other cells, facilitating the rolling and adhesion of leukocytes during the inflammatory response. The study of L-selectin/CD62L recombinant proteins has garnered significant attention due to their potential applications in understanding immune system dynamics and developing therapeutic strategies for various diseases, including autoimmune disorders, allergies, and cancer. Researchers have focused on the structure-function relationship of L-selectin, investigating how its various domains contribute to its adhesion properties. Additionally, recombinant L-selectin proteins serve as valuable tools in experimental settings, allowing for the study of its interactions with ligands and the design of inhibitors that could modulate immune responses. The generation of these recombinant proteins has enabled detailed investigations into the mechanisms of leukocyte trafficking and has opened avenues for novel therapeutic interventions aimed at modulating immune cell behavior in different pathological contexts.
