Analytical Data
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基因名
PD-1
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简介
PD-1 (programmed cell death 1) protein negatively regulates immune responses and affects processes such as apoptosis and tolerance induction. It is a transmembrane protein found on the outside of the plasma membrane and expressed in the retina. PD-1 Protein, Mouse (HEK293, His-Fc) is the recombinant mouse-derived PD-1 protein, expressed by HEK293 , with C-hFc, C-8*His labeled tag.
- Application
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生物活性
1. Determined by its ability to prevent plate adhesion of PHA-stimulated Jurkat cells in the presence of 625 ng/mL of bound hPD-L1. The ED50 for this effect is 0.4262 μg/mL, corresponding to a specific activity is 2.35×103 units/mg. 2. Immobilized Recombinant Mouse PD-1 at 1 μg/ml (100 μl/well) can bind Recombinant Mouse PD-L1. The ED50 for this effect is 0.5001 μg/mL. Determined by its ability to prevent plate adhesion of PHA-stimulated Jurkat cells in the presence of 625ng/mL of bound hPD-L1. The ED50 for this effect is 0.4262 μg/mL, corresponding to a specific activity is 2.35×103 units/mg.
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别名
Programmed cell death protein 1; PD1; CD279; PDCD1
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种属
Mouse
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表达系统
HEK293
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标签
C-hFc;C-8*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
NP_032824.1
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表达区间
L25-Q167
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蛋白长度
Partial
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分子量
60-65 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Programmed cell death protein 1 (PD-1) is an immune checkpoint receptor that plays a crucial role in regulating the immune system and maintaining self-tolerance. Its interaction with programmed death-ligand 1 (PD-L1) and PD-L2 negatively regulates T-cell activation and promotes tumor immune evasion, making it a significant target in cancer immunotherapy. The study of PD-1 recombinant proteins has gained momentum due to their potential in developing therapeutic agents that can enhance anti-tumor immunity. PD-1 blockade has shown promising results in various cancers, leading to the approval of several monoclonal antibodies targeting PD-1 or PD-L1, such as pembrolizumab and nivolumab. Researchers are increasingly focusing on the structural and functional characterization of PD-1 to enhance our understanding of its mechanisms and to improve the efficacy and safety of PD-1-targeted therapies. Moreover, ongoing studies are investigating the combination of PD-1 inhibitors with other treatment modalities, including chemotherapy, targeted therapy, and radiation, to overcome resistance and increase response rates. The exploration of PD-1’s role in other diseases, such as autoimmune disorders and chronic infections, further highlights its relevance in both therapeutic and diagnostic applications, underlining the critical importance of PD-1 recombinant proteins in contemporary biomedical research.
