Analytical Data
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Gene name
FLT3
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简介
FLT3, a receptor tyrosine kinase, has diverse functions including kinase activation, PI3K binding, and ligase binding. It plays a vital role in hematopoiesis, immune function, and tissue development. FLT3 is expressed in various tissues like the brain, immune system, and reproductive system. It is involved in diseases like leukemia and cancer. The wide functions and tissue-specific expression emphasize FLT3's importance in health and disease. FLT3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FLT3 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized human Flt-3 Ligand, at 1 μg/mL (100 μL/well) can bind Biotinylated Mouse FLT3 protein. The ED50 for this effect is 5.987 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized human Flt-3 Ligand, at 1 μg/mL (100 μL/well) can bind Biotinylated Mouse FLT3 protein. The ED50 for this effect is 5.987 ng/mL.
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Alternative Names
CD135; FL cytokine receptor; FLK2; Flk-2; Flt-3; STK-1
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Species
Mouse
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Source
HEK293
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q3UEW6
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Expression Region
N28-S544
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Protein Length
Partial
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Molecular Weight
88-100 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
FLT3 (Fms-like tyrosine kinase 3) is a crucial receptor tyrosine kinase that plays a significant role in hematopoiesis and the development of various blood cells, particularly in the context of acute myeloid leukemia (AML). Mutations in the FLT3 gene, particularly the internal tandem duplication (ITD) and point mutations in the tyrosine kinase domain, are prevalent in a substantial subset of AML cases and are associated with poor prognosis. Given its vital role in cell proliferation and survival, FLT3 has emerged as a key therapeutic target in cancer treatment. Researchers have been focusing on developing FLT3 inhibitors to improve clinical outcomes for AML patients. The study of recombinant FLT3 proteins facilitates a deeper understanding of FLT3's structure, function, and the mechanisms by which mutations lead to leukemogenesis. Additionally, recombinant FLT3 proteins are instrumental in the design of targeted therapies, biomarker development, and in vitro assays that help evaluate the efficacy of potential FLT3 inhibitors. Overall, the ongoing research into FLT3 and its recombinant proteins not only enhances our understanding of AML pathology but also accelerates the development of innovative treatments aimed at targeting FLT3-driven malignancies.
