Analytical Data
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Gene name
CD59
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简介
The CD59 protein is a potent inhibitor of the complement membrane attack complex (MAC) and exerts regulatory control at or after the C5b-8 stage.Its critical role makes CD59 a key regulator in the complement system, preventing overactivation and associated membrane damage.CD59 Protein, Rat (HEK293, His) is the recombinant rat-derived CD59 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human C9 is present at 5 μg/mL, can bind Recombinant Rat CD59. The ED50 for this effect is 2.01 μg/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human C9 is present at 5 μg/mL, can bind Recombinant Rat CD59. The ED50 for this effect is 2.01 μg/mL.
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Alternative Names
CD59 glycoprotein; HRF-20; MAC-IP; MACIF; MIRL; CD59; MIC11; MIN1; MSK21
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Species
Rat
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P27274
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Expression Region
L23-N100
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Protein Length
Partial
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Molecular Weight
14-16 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD59, a glycosylphosphatidylinositol (GPI)-anchored membrane protein, plays a crucial role in the regulation of the complement system, which is a vital part of the immune response. It inhibits the formation of the membrane attack complex (MAC), thereby protecting host cells from lysis by complement proteins. Abnormal expression or dysfunction of CD59 has been linked to various pathologies, including autoimmune diseases, cancer, and infectious diseases. Consequently, researchers have focused on the potential therapeutic applications of CD59, exploring its role not only in complement regulation but also in modulating immune responses. Recombinant CD59 proteins are being developed to better understand its functions and mechanisms, to design novel therapies that can enhance immune protection in patients with compromised immune systems, or to inhibit complement activation in conditions characterized by excessive complement activity. Additionally, CD59 is being investigated as a possible target for drug delivery systems and vaccine development. The study of CD59 recombinant proteins also provides insights into the structure-function relationships of membrane proteins, advancing our understanding of cell biology and immunology. Overall, the research on CD59 recombinant proteins holds promise for innovative therapeutic solutions while contributing to the broader field of immunology and molecular biology.
