Analytical Data
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Gene name
CD44
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简介
CD44 Protein is a type of cell surface receptor protein. CD44 Protein mediates various signaling pathways, including protein kinases, changes in the cytoskeleton, intracellular pathways, proteases, and transcription factors, which promote cancer cell division, proliferation, invasion, angiogenesis, and metabolic changes. The expression level of CD44 Protein is positively correlated with the malignancy and invasiveness of glioblastoma. CD44 Protein, Human (HEK293, His) is a recombinant CD44 protein tagged with a C-6*His label, expressed by HEK293. CD44 Protein, Human (HEK293, His) consists of 200 amino acids and has a molecular weight of 38-50 kDa[1][2].
- Application
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Biological Activity
Measured in a cell proliferation assay using A549 cells. The ED50 this effect is 1.543 ng/ml, corresponding to a specific activity is 6.481×105 units/mg. Measured in a cell proliferation assay using A549 cells. The ED50 for this effect is 1.543 ng/mL, corresponding to a specific activity is 6.481×105 units/mg.
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Alternative Names
CD44; CDw44; Epican; ECMR-III; LHR; MDU2; MDU3; MIC4; PGP-I; CSPG8; HCELL; HUTCH-I; IN; MC56
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Species
Human
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P16070-1
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Expression Region
Q21-P220
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AA Sequence
QIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIP
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Protein Length
Partial
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Molecular Weight
38-50 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD44 is a cell surface glycoprotein that serves as an integral component in various biological processes, including cell-cell interactions, cell adhesion, and signaling. It exists in multiple isoforms generated through alternative splicing, which allows for diverse functional roles in different tissues. CD44 is well-known for its role in inflammation, immunity, and cell migration, particularly in cancer metastasis, as it facilitates the adhesion of tumor cells to the extracellular matrix and lymphatic endothelium. This makes CD44 a significant target for therapeutic strategies in cancer treatment and regenerative medicine. Recent advancements in biotechnology have enabled the development of recombinant CD44 proteins, which provide valuable tools for studying its structure, function, and interactions with ligands such as hyaluronic acid. Research has increasingly focused on characterizing the various isoforms of CD44 to understand their specific roles in disease processes and to explore their potential as biomarkers for cancer prognosis and therapy response. The production of recombinant CD44 proteins can also aid in drug delivery systems and the design of novel therapeutic agents, making CD44 a key player in both basic and applied biomedical research.
