Recombinant Mouse PD-1 Protein(HEK293), His

PD-1

PD-1 Protein is an important immune regulatory molecule expressed on the surface of activated immune cells. By binding to its ligands (PD-L1/PD-L2), the PD-1 Protein exerts immunosuppressive effects. PD-1 Protein can be used in the research of tumors, autoimmune diseases, and other conditions. PD-1 Protein, Mouse (HEK293, His) is a recombinant PD-1 protein expressed by HEK293 cells and carries a C-6*His tag and glycosylation modification[1][2].

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

1.Determined by its ability to prevent plate adhesion of PHA-stimulated Jurkat cells in the presence of 625 ng/mL of bound hPD-L1. The ED50 for this effect is <1 μg/mL. 2.Immobilized Mouse PD-1 at 1 μg/ml (100 μl/well) can bind Biotinylated Mouse PD-L1, Fc (HY-P70633). The ED50 for this effect is <1 μg/mL. 3.Immobilized Mouse PD-1 at 1 μg/mL (100 μL/well) can bind Biotinylated Mouse PD-L1, His (HY-P70632). The ED50 for this effect is <1 μg/mL. Determined by its ability to prevent plate adhesion of PHA-stimulated Jurkat cells in the presence of 625ng/mL of bound hPD-L1. The ED50 for this effect is 0.986 μg/mL, corresponding to a specific activity is 1.01×103 units/mg.

Programmed cell death protein 1; PD-1; CD279; Pdcd1; mPD-1

Mouse

HEK293

C-6*His

Greater than 90% as determined by SDS-PAGE.

Q02242

L25-Q167

Extracellular Domain

25-43 kDa.

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.