Analytical Data
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Gene name
Siglec-10
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简介
Siglec-10 protein is thought to be a putative adhesion molecule that mediates sialic acid-dependent cell binding, preferentially selecting α-2,3- or α-2,6-linked sialic acid. Its sialic acid recognition site may be masked by cis interactions. Siglec-10 Protein, Human (Biotinylated, HEK293, Fc-Avi) is the recombinant human-derived Siglec-10 protein, expressed by HEK293 , with C-Avi, C-hFc labeled tag.
- Application
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Alternative Names
MGC126774; PRO940; Siglec10; SLG2; sialic acid-binding Ig-like lectin 10; siglec-like gene 2
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Species
Human
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Source
HEK293
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Tag
C-Avi;C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96LC7-1
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Expression Region
M17-T546
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AA Sequence
MDGRFWIRVQESVMVPEGLCISVPCSFSYPRQDWTGSTPAYGYWFKAVTETTKGAPVATNHQSREVEMSTRGRFQLTGDPAKGNCSLVIRDAQMQDESQYFFRVERGSYVRYNFMNDGFFLKVTALTQKPDVYIPETLEPGQPVTVICVFNWAFEECPPPSFSWTGAALSSQGTKPTTSHFSVLSFTPRPQDHNTDLTCHVDFSRKGVSAQRTVRLRVAYAPRDLVISISRDNTPALEPQPQGNVPYLEAQKGQFLRLLCAADSQPPATLSWVLQNRVLSSSHPWGPRPLGLELPGVKAGDSGRYTCRAENRLGSQQRALDLSVQYPPENLRVMVSQANRTVLENLGNGTSLPVLEGQSLCLVCVTHSSPPARLSWTQRGQVLSPSQPSDPGVLELPRVQVEHEGEFTCHARHPLGSQHVSLSLSVHYSPKLLGPSCSWEAEGLHCSCSSQASPAPSLRWWLGEELLEGNSSQDSFEVTPSSAGPWANSSLSLHGGLSSGLRLRCEAWNVHGAQSGSILQLPDKKGLIST
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Protein Length
Extracellular Domain
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Molecular Weight
100-130 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Siglec-10 is a member of the Siglec (sialic acid-binding immunoglobulin-like lectin) family, which plays a crucial role in the immune system by recognizing sialic acid-containing glycoproteins and glycolipids. It is primarily expressed on immune cells, particularly in myeloid lineage, and is implicated in the regulation of immune responses, including the modulation of inflammation and the attenuation of autoimmune diseases. Research has shown that Siglec-10 can inhibit the activation of immune cells, such as macrophages and dendritic cells, thereby maintaining immune homeostasis. The significance of Siglec-10 in various pathological conditions, including cancer, has sparked interest in the development of therapeutic strategies targeting this molecule. Recombinant Siglec-10 proteins have been generated for studying its structure and function, allowing researchers to explore its potential as a biomarker for disease diagnosis and as a target for immunotherapies. Understanding the mechanisms by which Siglec-10 interacts with its ligands can provide insights into its role in health and disease, paving the way for novel treatment approaches that leverage its immune-regulatory functions. This growing body of research emphasizes the importance of characterizing Siglec-10, particularly in the context of its contributions to immune regulation, making it a promising area for future biomedical investigation.
