Analytical Data
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Gene name
Jagged-1/JAG1
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简介
Jagged-1/JAG1 protein is a ligand of multiple Notch receptors, mediates Notch signaling, affects cell fate decisions during hematopoiesis and enhances angiogenesis. It functions throughout mammalian cardiovascular development, regulates myoblast differentiation, and participates in the complex Notch signaling network through interactions with NOTCH1, NOTCH2, and NOTCH3. Jagged-1/JAG1 Protein, Rat (HEK293, His) is the recombinant rat-derived Jagged-1/JAG1 protein, expressed by HEK293.
- Application
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Biological Activity
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1.264 μg/mL, corresponding to a specific activity is 791.1392 units/mg. Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1.264 μg/mL, corresponding to a specific activity is 791.1392 units/mg.
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Alternative Names
AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1
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Species
Rat
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q63722
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Expression Region
S32-D1067, with substitutions GGARN56-60AE and DR63-64TLVRPY
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Protein Length
Partial
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Molecular Weight
125-130 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Jagged-1 (JAG1) is a crucial ligand in the Notch signaling pathway, which plays a pivotal role in various biological processes, including cell differentiation, proliferation, and apoptosis. Dysregulation of Notch signaling, often due to aberrant expression of JAG1, is implicated in numerous diseases, including cancer and developmental disorders. The study of Jagged-1 has gained significant interest in recent years as researchers investigate its structure and functional mechanisms to better understand its role in cellular communication and tissue development. Recombinant JAG1 proteins are commonly produced to facilitate this research, allowing scientists to explore its interaction with Notch receptors and the subsequent signaling outcomes. By utilizing advanced protein expression systems, such as mammalian cell lines, researchers can generate biologically active forms of JAG1, which can then be used in various assays to elucidate its functional properties. These studies not only enhance our understanding of the fundamental biology of JAG1 but also hold potential therapeutic implications for diseases linked to the Notch pathway. The continued research into Jagged-1 and its recombinant protein forms underscores the importance of this ligand in both developmental biology and disease pathology, paving the way for novel therapeutic strategies targeting Notch signaling dysfunctions.
