Redefining the Speed and Possibilities of Recombinant Protein Production
Ipodix leverages over a decade of synthetic biology research to introduce the CellFreeX™ cell-free expression system, breaking the limitations of traditional cell culture and achieving full-cycle production from gene to purified protein within 3 days. This innovation provides new tools for drug discovery, diagnostic development, and beyond.
Technical Advantages
1. Ultra-Fast Expression
● Protein synthesis in 6 hours (vs. 3–6 weeks for CHO systems).
● 24-hour yields of 2–5 mg/mL (5–8x higher than E. coli systems).
2. Complex Protein Handling
● >90% success rate for toxic proteins (e.g., membrane proteins, proteases).
● Supports disulfide bond formation (>85% correct folding) and glycosylation (92% similarity to HEK293 glycoforms).
3. Flexible Open System
● Compatible with non-natural amino acids and isotope labeling (>95% efficiency for 15N/13C).
● Accepts linear DNA templates (no cloning required).
4. Industrial Scalability
● Proprietary continuous-flow reactor enables >10 g/batch.
● 80% lower production costs vs. mammalian systems.
5. Rigorous Quality Control
● Endotoxin levels <0.1 EU/μg.
● >99% sequence coverage verified by mass spectrometry.
6. Sustainability
● 70% reaction system recycling rate.
End-to-End Services: From Sequence to Purified Protein
Standardized and Custom Solutions
![Service Workflow: Demand Analysis (24h) → Template Design (8h) → Reaction Optimization (24h) → Protein Synthesis (6–24h) → Purification & Validation (24h) → Delivery]Key Innovations
● AI-Powered Template Design: Optimized UTR sequences boost translation efficiency by 300%.
● Four Base Kits: Prokaryotic, eukaryotic, plant-type, and thermostable systems, customizable with 20+ chaperones/foldases.
● Nanobody Directed Evolution: Achieve 3 rounds of mutation screening in 2 weeks via mRNA display.
Delivery Standards
● Basic: SDS-PAGE purity >90%, concentration ≥1 mg/mL.
● Advanced: HPLC purity >95% with activity reports.
● GMP Grade: USP <1043> compliance and full CMC documentation.
Applications: Accelerating Life Science Breakthroughs
1. Drug Discovery & Optimization
● Rapid screening of 50 scFv variants per cycle.
● Case: ADC drug toxin-antibody conjugation analysis completed in 3 days (vs. 2 months traditionally).
2. Vaccine Development
● 85% VLP assembly efficiency.
● Case: COVID-19 RBD antigen production from gene synthesis to animal trials in 11 days.
3. IVD Reagent Development
● Labeling enzymes (HRP/ALP) with specific activity >5000 U/mg.
● Case: Chemiluminescent reagent antigen production with CV <2% batch variation.
4. Enzyme Engineering
● High-throughput mutant library screening (1000 variants/day).
● Case: Industrial lipase thermal stability increased by 20°C.
5. Personalized Medicine
● Tumor neoantigen synthesis delivered in 72 hours.
6. ● Case: Patient-specific mutant protein for T-cell therapy development.Research & Education
● Lyophilized kits with 6-month room-temperature stability.
● >95% success rate in teaching labs.
Success Stories: A Global Efficiency Revolution
Case 1: Top 10 Pharma’s mRNA Vaccine Development
● Challenge: Urgent need for Spike protein production.
● Solution: CellFreeX eukaryotic kit.
● Result: Active protein obtained in 3 days, accelerating clinical trials by 8 weeks.
Case 2: Biotech’s Nanobody Development
● Challenge: Camelid antibody expression in mammalian cells.
● Solution: Cell-free system + directed evolution.
● Result: pH-stable nanobody with pM-level KD value.
Case 3: IVD Company’s Multi-Species Serum Protein Production
● Challenge: Parallel production of 8 animal-derived proteins.
● Solution: CellFreeX parallel expression.
● Result: 2-week completion of a 6-month workload, enabling faster product launch.
FAQs & Quality Commitment
Q1: How does protein activity compare to traditional systems?
● Third-party validation: EGFR antibody binding activity shows <5% deviation from HEK293 products.
Q2: Can complex multi-transmembrane proteins be expressed?
● GPCRs with >80% activity achieved via lipid-assisted membrane environment simulation.
Q3: How are cost savings achieved at scale?
● In-house raw material production + continuous-flow process reduces costs to $50/mg for 10,000 mg batches.
Start Your Protein Revolution Today!
Submit your sequence to receive a free feasibility analysis within 24 hours.
→ First-time clients enjoy 10% off GMP-grade services.
→ Priority access to our AI-driven template design platform.
