Ipodix Biotech: High-throughput Recombinant Antibody Production Service
Recombinant antibodies, produced via genetic engineering, offer high specificity and batch consistency, making them indispensable tools for therapeutic development, precision diagnostics, and biological research. Leveraging high-throughput expression platforms and scalable production processes, Ipodix Biotech provides end-to-end services from gene synthesis to milligram/gram-scale antibody production, accelerating antibody drug R&D and industrialization.
Technology Platforms & Core Advantages
Ipodix employs mammalian (HEK293, CHO), insect, and yeast expression systems with automated screening and process optimization for high-efficiency antibody production:
● High-throughput processing: Simultaneous synthesis, vector construction, and expression validation for 100+ antibody genes per batch.
● High yield: Mammalian systems achieve 1–5 g/L antibody titers with >95% purity.
● Format flexibility: Produces full-length IgG, Fab fragments, scFv, bispecific antibodies, and more.
● Rapid delivery: 4–6 weeks from gene to purified antibody, scalable from mg to kg quantities.
Workflow & Timelines
| Gene Synthesis & Optimization | 1–2 weeks | Codon-optimized gene sequence, sequencing report. |
| Expression Vector Construction | 1–2 weeks | Recombinant plasmid, transfection efficiency data. |
| Small-scale Expression & Optimization | 2–3 weeks | Expression level analysis, purification protocol. |
| Large-scale Production & Purification | 2–4 weeks | High-purity antibodies (≥95%), QC certificates. |
Expression System Selection Guide
| Mammalian Cells | Full glycosylation, high-activity antibodies. | Therapeutic antibodies, diagnostic reagents. |
| Insect Cells (Sf9) | Cost-effective for complex proteins. | Membrane proteins, viral antigens. |
| Yeast Systems | Rapid production, no glycosylation. | Research antibodies, in vitro diagnostics. |
Applications
| Antibody Drug Development | Cancer immunotherapeutics, bispecific antibodies, ADCs. |
| In Vitro Diagnostics | Chemiluminescence/immunoassay reagent production. |
| Cell Therapy | CAR-T/CAR-NK targeting scFv fragments. |
| Research Tools | Epitope mapping, protein interaction studies. |
| Industrial Enzyme Engineering | High-throughput screening for engineered antibodies. |
Quality Assurance
Ipodix adheres to cGMP-like standards for drug-ready antibodies:
● Purity testing: SDS-PAGE, SEC-HPLC (≥95% purity).
● Activity validation: ELISA/SPR (KD ≤1 nM), FACS-based cell binding.
● Safety: Host protein residues <0.1%, endotoxin <1 EU/mg.
● Stability: Stress testing (heat, light, freeze-thaw) and long-term storage.
Collaboration Process
Requirement Confirmation: Submit antibody sequence, expression system, and scale.
Protocol Design: Receive technical proposal and quote within 24 hours.
Process Development: Optimize expression and complete pilot production.
Scale-Up Production: Batch production in 50L–2000L bioreactors.
Delivery & Validation: Antibody samples, process documentation, and QC reports.
Service Highlights
● Custom Modifications: Fc engineering, site-specific conjugates (e.g., fluorescent labels, biotinylation).
● Cost Optimization: Platform processes reduce production costs by 30%.
Why Choose Ipodix?
● Technical Expertise: 500+ delivered projects, including PD-1, VEGF, and other high-value targets.
● Expedited Timelines: Priority scheduling cuts project cycles by 20%.
● Post-Service Support: 6 months of free technical consultation and process refinement.
Accelerate Your Antibody Development Journey!
Contact Ipodix experts via our website or hotline to discuss your project.
