Ipodix Biotech: Single B Cell Antibody Service
Single B cell antibody technology isolates antigen-specific B cells and clones their antibody genes to efficiently obtain naturally paired heavy-light chain antibodies, making it ideal for developing high-affinity, high-specificity antibodies. Ipodix Biotech leverages flow cytometry sorting, microfluidic chips, and single-cell sequencing to deliver fully human or murine monoclonal antibody services for vaccine research, cancer immunotherapy, and infectious disease applications.
Technical Advantages
Ipodix combines cutting-edge single-cell technologies with antibody engineering expertise to ensure efficiency and quality:
● High-specificity sorting: Precise isolation of antigen-positive B cells via flow cytometry or antigen-labeled magnetic beads.
● Native pairing retention: Direct retrieval of VH/VL genes from single cells, avoiding chain loss in library construction.
● Rapid gene recovery: Patented primer design achieves >95% antibody gene amplification success rate.
● Flexible sourcing: Supports B cells from blood, spleen, lymph nodes, and multiple species (human, mouse, primate).
Workflow & Timelines
| Sample Processing & B Cell Sorting | 1–2 weeks | Sorted cell count report, antigen-binding activity data. |
| Single-Cell Gene Amplification | 2–3 weeks | VH/VL gene sequences, sequencing QC report. |
| Recombinant Antibody Expression & Screening | 3–4 weeks | Candidate antibody sequences, KD affinity data. |
| Functional Validation (Optional) | 2–4 weeks | Neutralization activity, epitope mapping, or cross-reactivity analysis. |
Platform Features
| Single B Cell Sorting | ≥90% capture rate for antigen-positive cells, ≥95% purity. |
| Heavy/Light Chain Amplification | ≥85% full-length gene recovery success rate. |
| Recombinant Antibody Expression | Mammalian system expression, ≥90% purity. |
| Antibody Screening | 1000+ candidates screened per batch. |
Applications
| Anti-Infective Drug Development | Bacteria/virus-specific antibody discovery. |
| Antibody Engineering | Mining natural antibody libraries, humanization. |
| Cancer Immunotherapy | Tumor-targeting antibody development. |
| Autoimmune Disease Research | Pathogenic autoantibody characterization. |
Service Highlights
High-Efficiency Targeted Sorting
a. Multicolor fluorescence labeling and magnetic bead pre-enrichment for rare B cells (e.g., memory B cells, plasma cells).
b. Supports membrane protein (GPCRs, ion channels) and multi-epitope antigen screening.Automated Gene Cloning
a. Single-cell isolation via microfluidic chips or 96-well plates to minimize cross-contamination.
b. Nested PCR for antibody gene amplification, compatible with IgG/IgM/IgA subtypes.Functional Validation Systems
a. Pseudovirus neutralization assays, FACS-based binding activity, and epitope competition analysis.
b. Antibody humanization and affinity maturation (KD optimization to pM-level).
Quality Assurance
Ipodix enforces rigorous quality control throughout antibody development:
● Sequence validation: Sanger sequencing confirms VH/VL gene accuracy.
● Expression verification: ELISA/Western Blot validates antibody secretion.
● Affinity assessment: SPR/BLI measures KD values (sensitivity: 0.1 nM).
● Batch consistency: ≤15% variability in expression for antibodies from the same B cell.
Collaboration Process
Requirement Consultation: Provide antigen details, sample type, and antibody specifications.
Protocol Design: Receive sorting strategy and proposal within 72 hours.
Sample Submission: Accepts frozen cells or tissues (must meet biosafety standards).
Project Execution: Regular updates on sorting efficiency, gene amplification, and screening.
Delivery: Antibody sequences, expression plasmids, or purified antibodies (optional).
Why Choose Ipodix?
● Proven Expertise: 300+ completed projects across 20+ disease targets.
● Cost-Effective: 40% lower cost per antibody compared to hybridoma technology.
● Fast Turnaround: Standard timeline 8–12 weeks; expedited projects in 6 weeks.
● End-to-End Support: From B cell sorting to humanization and scale-up.
● Data Transparency: Raw sequencing data, expression curves, and functional validation reports.
Transform B Cells into Precision Antibodies!
Contact the Ipodix technical team via our website or hotline to discuss your project.
