Ipodix Insect Baculovirus Expression System: A Precision Platform for Eukaryotic Protein Production
Technical Advantages: Six Breakthrough Features Beyond Mammalian Systems
Ipodix’s third-generation recombinant baculovirus technology enables efficient expression and precise post-translational modification of complex proteins, meeting both drug development and industrial production demands.
1. High Secretion Efficiency
● 70–90% extracellular secretion rate, significantly reducing purification complexity
● Yield of 5–800 mg/L per infection cycle (3–8× higher than mammalian systems)
2. Accurate Post-Translational Modifications
● Glycosylation profile achieves 75% similarity to mammalian systems
● >98% disulfide bond accuracy and >90% phosphorylation precision
3. Multi-Gene Co-Expression Capability
● Single vector supports simultaneous expression of 4 foreign genes
● Case: Successful production of Fc-fused trimeric protein complexes
4. Serum-Free Culture Compatibility
● Compatible with SF900 III/SFM suspension media
● Cell density up to 8×10⁶ cells/mL (10× higher than adherent cultures)
5. Biosafety Assurance
● Non-infectious to vertebrates (compliant with lab safety standards)
● Endotoxin-free production
6. Scalable Manufacturing
● Single 500L bioreactor batch yields 200–500 g
● Production costs reduced by 40–60% vs. HEK293 systems
Service Workflow: End-to-End Development from Gene to Product
Ipodix’s 14-step standardized process ensures >88% project success rate.
Stage 1: Gene Design & Vector Construction (5–7 days)
● Codon optimization covering 12 lepidopteran insect preferences
● Dual polyhedrin (polh) and p10 promoter coordination
Stage 2: Viral Recombination & Screening (8–10 days)
● >99% recombination efficiency (Tn7 transposon system)
● Plaque assay-purified virus with titer >1×10⁸ PFU/mL
Stage 3: Cell Culture & Infection (7–14 days)
● MOI optimization range: 0.1–10
● Real-time cell viability monitoring (harvest triggered at >85% viability)
Stage 4: Downstream Purification (5–7 days)
● Three-step chromatography: affinity → ion exchange → size exclusion
● Ultrafiltration concentration system (final concentration 0.1–50 mg/mL)
Stage 5: Quality Validation & Delivery
● Basic QC: SDS-PAGE, Western Blot, HPLC
● Advanced QC: Glycan profiling (mass spectrometry), bioactivity validation
Applications: Spanning the Biopharma Value Chain
1. Cell Therapy Proteins
● CAR-T cell adaptor proteins with >97% correct folding rate
● Case: Bispecific antibody yield boosted to 1.2 g/L
2. Custom Research Reagents
● 8 tagging systems (His, GST, etc.)
● Lyophilized formulations with >36-month ambient stability
Success Stories: Global Industrial Validation
Biopharma Collaboration
▷ Client: A CAR-T therapy company
● Challenge: Express a 4-transmembrane domain adaptor protein
● Result: High-activity protein achieved (EC50 = 0.3 nM)
Academic Partnership
▷ Client: A university structural biology center
● Goal: Resolve 3D structure of a G protein-coupled receptor
● Result: 2.5Å resolution cryo-EM data published in Cell
Quality System: Full-Cycle Compliance with International Standards
Process Control
● Cell viability fluctuation <±3%
● Temperature deviation <±0.3°C during culture
Delivery Specifications
● Standard Grade: >90% purity (SDS-PAGE), endotoxin <1 EU/mg
● GMP Grade: ICH Q5D compliance, host protein residue <10 ppm
Client Benefits
● Full SOP and process validation documentation transfer
● Complimentary 12-month stability monitoring
Why Choose Ipodix?
☑ 1,200+ baculovirus expression projects completed
☑ Served clients in 30 countries
Start Your Project Today
1. Submit protein sequence to receive process development proposal within 24 hours.
2. Free codon optimization service.
3. Priority access to pilot production slots.
