Ipodix Bacillus Expression System: An Industrial-Grade Solution for High-Efficiency Secretory Expression in Gram-Positive Bacteria
I. Technical Advantages: Five Core Capabilities Breaking Traditional Limits
Leveraging the natural secretory properties of Bacillus subtilis, Ipodix has developed a technological platform that delivers a highly efficient and cost-effective production solution for industrial enzymes, antimicrobial peptides, and other proteins.
1. Exceptional Secretion Capacity
● Extracellular secretion efficiency >80%, with target proteins directly released into the culture medium
● Maximum fermentation density of 50 g/L (DCW), recombinant protein yield >5 g/L
2. Rapid Production Cycle
● Gene-to-fermentation completion in just 7–10 days
● Fed-batch fermentation cycle controlled within 24–36 hours
3. Stringent Biosafety
● Non-pathogenic GRAS-certified strain (FDA 21 CFR 184 compliant)
● Endotoxin-free products suitable for food-grade applications
4. Low-Cost Scalability
● Media costs reduced to 1/3 of E. coli-based systems
● Single-batch output in 15-ton fermenters meets ton-scale demands
5. Complex Protein Compatibility
● Successfully expressed antimicrobial peptides with 8 disulfide bonds
● Correct folding rate of secreted proteins >90%
II. Service Workflow: End-to-End Development from Gene Design to Industrial Scaling
Ipodix’s standardized development process maintains an industry-leading project success rate of >85%:
Stage 1: Gene Optimization & Vector Construction (3–5 days)
• Codon optimization compatible with 15 industrial Bacillus subtilis strains
• Promoter library selection (P43, Pgrac, PamyQ, etc.)
Stage 2: Transformation & Strain Screening (5–7 days)
• High-efficiency electroporation (>1×10⁶ CFU/μg DNA)
• Dual antibiotic markers for rapid screening
Stage 3: Fermentation Process Development (7–10 days)
• Scale-up from shake flasks to 500L fermenters
• Real-time monitoring of dissolved oxygen, pH, biomass, and critical parameters
Stage 4: Product Harvest & Purification (3–5 days)
• High-density broth processing via continuous-flow centrifugation
• Three-step purification: ultrafiltration → ion exchange → gel filtration
Stage 5: Quality Verification & Delivery
• Basic testing: SDS-PAGE purity, concentration determination
• Advanced testing: Mass spectrometry (MW validation), bioactivity assays
III. Applications: Full-Spectrum Solutions for Industrial and Pharmaceutical Needs
1. Research Protein Production
● Intracellular/secretory expression modes available
● Lyophilized formulations with >18-month ambient stability
IV. Success Stories: Globally Validated Industrial Solutions
Collaboration with Research Institutions
• Feed Research Institute, Chinese Academy of Agricultural Sciences
● Requirement: Thermostable β-mannanase for high-temperature applications
● Outcome: Enzyme activity of 15,000 U/mg, half-life >2 hours at 80°C
● Technology awarded the Second Prize of National Science and Technology Progress
V. Quality System: Full-Cycle Control from Strain to Product
Strain Management
● Master Cell Bank validated via whole-genome sequencing
● Monthly genetic stability testing
Process Control
● Critical parameter deviations:
○ Fermentation pH fluctuation <±0.2
○ Temperature deviation <±0.5°C
Delivery Standards
● Basic Grade: Purity >85% (SDS-PAGE), enzyme activity error <±5%
● Food Grade: ISO 22000 compliance with allergenicity testing
● Pharmaceutical Grade: EP/USP standards, host protein residue testing
Customer Benefits
● Technology Transfer: Strain preservation and technical training
● After-Sales Support: 12 months of complimentary consultation
Why Choose Ipodix?
● 400+ Bacillus subtilis projects completed
● Clients across 25 countries
● >95% industrial-scale success rate
● Technical team with 12+ years of average industry experience
Act Now!
● Submit your protein sequence and receive a feasibility analysis within 24 hours.
● Free signal peptide screening service
● Priority access to pilot-scale production slots
